Digvijay Singh Yadav

5 Questions 4 Answers 0 Followers

Questions related from Digvijay Singh Yadav

What may be the reason that MDA-MB-231 cells do not get attached to the surface of tissue culture flasks?

Earlier I used to take out the cells (max 3 months old stored at -80C) quick thaw them by rubbing in between the palms and then put in 10% FBS after centrifuging it in complete media. and the...

01 January 2020 8,344 3 View

What is the specific use of normal and heat inactivated FBS in cell culture?

I do know that the heat inactivation will kill all the complement protiens and some cell lines may or may not grow better in inactivated FBS. How important is it? and how to know wether i need an...

02 February 2018 4,205 14 View

What are the 'must take' precautions for shade drying Algal biomass?

I collected the algae from the shore, removed any impurities and shells they were attached, let them hang in shade to remove excess water and spread them in shade to dry. Later i wish to powder...

02 February 2018 8,430 2 View

I wish to stain sections of algae (Specifically: order Ceramiales). Can anyone suggest an alternate stain for Aniline blue?

Centroceras and Ceramium are mostly stained using aniline blue.

01 January 2016 9,572 3 View

DNA shearing in gel electrophoresis, suggestions?

I have extracted DNA from different algal samples and after extraction of DNA i used various primers for DNA amplification. I got some good results (as clear & distinct bands) but for some I...

07 July 2014 2,990 10 View