At what point does UNG/UDG become noticeably detrimental in one-step RT-PCR for the purpose of diagnostics?

I noticed we've been using a PCR system in our one-step RT-PCRs that incorporates dUTPs and uracil-DNA glycosylase (UDG). The UDG is not the cod-derived version. Our reverse transcription (RT'n)...

05 November 2018 152 0 View