Diego Robles Mazzotti

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Questions related from Diego Robles Mazzotti

What is the best approach for statistical analysis of qPCR using the comparative Ct method?

I've been thinking about this for a while... Some use the raw deltaCT to perform statistics (t-test, ANOVA etc), others prefer to exponentiate and use 2^-deltaCT, while others report statistical...

12 December 2012 6,140 28 View

What is the best pre-processing (background correction + normalization + summarization) algorithm to use in miRNA microarray, using Affymetrix platform?

I've heard that RMA might not be applicable and some suggestions would be using VSN (as vsnrma in Bioconductor). Is that right?

09 September 2012 2,772 1 View