I'm doing high molecular weight western blot (above 200) but I'm doing low weight endogenous blot (37), how can I detect both?

Although I can detect the protein (above 200), leaving it for longer in the transfer (plus 2h), I think I am losing my endogenous protein, as its detection is not getting good. How can I solve it?

08 April 2024 2,190 3 View