P1 and E.17 mouse brains are fixed using 70% ethanol. How can I make my immunofluorescence staining works?

I got some brains (P1 and E.17 mouse brains) from a collaborator who already fixed them in formaldehyde in 2015, and then transferred to 70% ethanol for storage since then (almost 2 yrs). I...

08 March 2017 5,497 2 View