Diego Aguilar

2 Questions 5 Answers 0 Followers

Questions related from Diego Aguilar

How much further would a supercoiled plasmid migrate?

I'm validating if my clone was successful. I first made a colony PCR and the correct band appears. I was then told to validate further using restriction digestion. I added 15 uL of aprox 16 ng/uL...

01 November 2016 2,304 3 View

Why is my DNA insert into pET32b+ vector, not in frame?

I'm currently working with the expression vector pET32b+ (Novagen). I have been trying the insert a DNA sequence into the vector in sillco, without success. After trying once and again, I...

24 March 2016 5,313 6 View