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I'm validating if my clone was successful. I first made a colony PCR and the correct band appears. I was then told to validate further using restriction digestion. I added 15 uL of aprox 16 ng/uL...
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I'm currently working with the expression vector pET32b+ (Novagen). I have been trying the insert a DNA sequence into the vector in sillco, without success. After trying once and again, I...
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