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Questions related from Diego Aguilar
I'm validating if my clone was successful. I first made a colony PCR and the correct band appears. I was then told to validate further using restriction digestion. I added 15 uL of aprox 16 ng/uL...
01 November 2016 2,370 3 View
I have been trying to express my recombinant protein (15 kD) for some time now without success. I have tried @ 37 C, OD600 0.6 for 5 hours with 1mM IPTG and I have tried 32 C, OD600 0.5 for 4...
28 September 2016 144 9 View
I'm currently working with the expression vector pET32b+ (Novagen). I have been trying the insert a DNA sequence into the vector in sillco, without success. After trying once and again, I...
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