Didem Tecimel

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Questions related from Didem Tecimel

Have you ever experienced a successful transfection (depending on the marker gene and antibiotic selection) but no expression of GOI?

Both use lentiviral vector backbone plasmids and sleeping beauty transposons for a gene transfer experiment. Cells (NK and T cells) express the eGFP protein and survive through antibiotic...

15 December 2022 4,468 3 View

Is plasmid linearization a good option to provide the integration of a large insert size?

I have a plasmid that is designed for lentiviral packaging but the size of the plasmid is ~12 kb and facing some problems in the packaging step. I've been planning the cut the part that includes...

29 March 2021 1,822 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

22 February 2021 6,557 3 View