Diane Tshikudi

2 Questions 7 Answers 0 Followers

Questions related from Diane Tshikudi

Does anyone have an idea of why fibroblast cells can attached to a surface without differentiating (change of shape)?

I have cultured NIH 3T3 fibroblast cells on soft ligand-coated polyacrylamide gels. The polyacrylamide gels were (12% acrylamide 0.6% bis-acrylamide) coated with fibronectin (100 ug/ml). The cells...

06 June 2016 7,830 5 View

How do I initiate polyacrylamide gel polymerization while using low bis-acrylamide concentration?

I am trying to create a polyacrylamide gel at low bis-acrylamide concentration (

10 October 2014 5,244 9 View