Diana Villarreal-Huerta

4 Questions 1 Answers 0 Followers

Questions related from Diana Villarreal-Huerta

How to prepare a sterile 1 N NaOH solution for post-autoclaving media pH adjustment?

I need to maintain the pH of a yeast minimal medium (initial pH: 4.3; ammonium sulfate, glucose and YNB) at pH 7 for at least 30 h post-inoculation, without using buffers. Is there a way to...

30 June 2025 6,517 0 View

How to purify quality total RNA from yeast that have reached stationary phase?

When purifying total RNA from yeast that have grown on nitrogen-limited medium, it always appears more degraded when coming from stationary phase than from log phase. RNA coming from log phase...

10 April 2023 3,959 2 View

Bunsen burner or laminar flow hood for aseptic manipulation of yeast cultures?

In the latest edition (2016) of the Basic Practical Microbiology Manual from the Microbiology Society, all the aseptic procedures are carried on near a Bunsen burner. This is the common practice...

28 March 2023 4,924 6 View

Can I use genomic DNA to make standard curves for RT-qPCR?

I've read that I need cDNA standard curves to test primer efficencies. I'd like to know if I could use genomic DNA instead, since I've got too many different conditions from which different cDNAs...

28 November 2022 5,355 4 View