Diana Turcu

3 Questions 3 Answers 0 Followers

Questions related from Diana Turcu

HAP1 cell transfection with RNP complexes?

Hi, Does anyone have experience with transfecting HAP1 cells for genome editing with Cas9/gRNA complexes? Or are the HAP1 cells difficult to transfect? Thanks.

28 August 2020 6,186 1 View

Tips to grow a culture after one cell sorting of Hek293T

Hi all, I am doing CRISPR on Hek293T cells. After FACS, the one cell wells do not survive/grow only the wells were 50/100 cells were plated. Any tips to improve the survival rate for the one cell...

01 January 1970 6,198 5 View

Fiji error ROI manager is empty

Hi, I was using a lot a macro that measures on the overlay from ROI manager in the DAPI channel, the fluorescence intensity in two different channels (green and red). However, now it stopped with...

01 January 1970 4,109 0 View