Diahann A. M. Atacho

2 Questions 2 Answers 0 Followers

Questions related from Diahann A. M. Atacho

Does anyone know the optimal coating to use on glass coverslips for electrophysiology on primary neuronal culture?

I am currently incubating the glass coverslips with 0.1mg/mL Poly-d-lysine for 1 hour and then with collagen diluted 1:10 for 2 hours at 37 degrees. While the neurons stick fine for immuno, they...

15 October 2015 8,809 4 View

How long can I leave glutamate on a neuronal culture without inducing toxicity?

I want to do a serial dilution of 10uM, 100uM to 1mM of glutamic acid on my primary neuronal cultures in order to look at gene expression level changes over time (after exposure to glutamic acid)....

15 October 2014 5,504 6 View