B16F10 cells once passaged, gets adherent and turn into black round cells. I would like to know what is the issue with B16F10 cell culture?

I have procured B16F10 cells from NCCS Pune and maintained them in DMEM with 10% FBS. The cells received were confluent and healthy but after the a first passage, the adherent cells showed...

01 April 2022 7,085 2 View