Dhrubajyoti Mahata

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Questions related from Dhrubajyoti Mahata

What will be appropriate negative control for SIINFEKL peptide-H2kb complex for CD8 T cell activation reaction?

I want to check whether my SIINFEKL peptide-H2Kb complex is able to activate the peptide specific CD8 T cells of OT-I mouse. But I want to use a negative control for the reaction to confirm that...

12 December 2018 7,947 2 View

How to store the Ni-NTA column purified BirA enzyme?

I have purified the E. coli enzyme BirA using the Ni-NTA column purification method and eluted the protein in 350 mM imidazole, 20mM Tris (pH 8), 150mM NaCl solution. what should be the storage...

09 September 2018 8,579 5 View

What buffer is required for in vitro biotinylation with the help of BirA enzyme?

BirA ligase helps in conjugation of biotin to any biomolecule which has a "biotin tag" sequence (like Avi tag) in its C terminus.

05 May 2018 6,546 3 View