Dhiraj Kumar

4 Questions 20 Answers 0 Followers

Questions related from Dhiraj Kumar

How we can calculate mRNA copy number/cell from ddPCR data?

I have ddPCR (digital droplet PCR) data from two different samples (control and unknown) and ddPCR data indicate that unknown sample have higher copy number/uL. I would like to know copy...

28 June 2019 6,628 3 View

Why do cancer stem cells sometimes exhibit high proliferation in vitro but exhibit less proliferation when placed in vivo in a tumor condition?

When i have checked the proliferation marker (Cyclin D1) in cancer stem cell (CSC) then its expression was higher. However when i have developed the in vivo tumor which is highly aggressive and...

22 December 2014 6,350 9 View

Is it cancer cell lines like MDA-MB 231, MCF7, and B16F10 that have stem cell properties?

Like in many paper researchers have claimed that cancer cell lines have cancer stem cell properties. However, cancer cell line is a clonal population of cells then how has it acquired stem cell...

14 July 2013 932 1 View

I want to chek cell viability but problem is that after FACS sorting i am getting very less no of cells (2000-3000) that is not enough to count on hem...

I am try to sort stem cell tumor sample and its no is very less and these sorted fraction i want to reinject in mice for that i need to chek cell viability.

03 September 2011 8,761 1 View