I am trying to express a protein fused with APEX2 in HEK293T cells. The vector control is expressing but the fusion protein is not expressing?

I tried and cloned the gene in the 3xFLAG-APEX2-NES vector. i am transfecting 1.5ug of each plasmid in HEK293T cells in 12 well plates at 80% confluency then after 48 hrs of transfection I...

24 August 2023 6,354 2 View