Dhaval Bhatt

2 Questions 3 Answers 0 Followers

Questions related from Dhaval Bhatt

How to increase the efficiency of transferring large molecular weight proteins ~265 kDa for western blot analysis?

I am trying to analyse acetyl CoA carboxylase (ACC) protein (~265 kDa) using Biorad Criterion gels and Nitrocellulose membranes. I have already tried separating it on a 7.5% gel with 4% stacking...

06 June 2013 9,562 8 View

How does a qRTPCR efficiency of more than 100% affect the interpretation or analysis of results?

I have just started doing qRTPCR using SYBR green (Roche) with ready made primers for the gene of interest (SABiosciences). I consistently find a reaction efficiency of greater than 100 in the...

02 February 2013 378 4 View