Dhaval Patel

6 Questions 31 Answers 0 Followers

Questions related from Dhaval Patel

If concentration of substrate and inhibitor is kept same in case of competitive inhibition, what will be the scenario?

If the concentration of substrate and inhibitor is kept the same in case of competitive inhibition, which molecule will bind to protein?

29 January 2020 4,010 4 View

Can we study protein interaction with heparin using fluorescence quenching spectroscopy studies?

I am working on a protein which has a affinity for complex sugars and for the same I need to study its biophysical properties and interaction with heparin using fluorescence spectroscopy. Heparin...

07 April 2016 4,619 3 View

Any other suitable method than gel filtration for protein molecular weight determination?

My protein of interest is naturally found as tetramer as majority species and sometimes as a dimer. Its mol. wt. is 39kDa. I have performed gel filtration experiment using S200 column from GE...

28 March 2016 4,854 18 View

How can I separate sugars from glycoprotein?

I am working on protein which binds with glycoprotein (mucin) via sugar moieties on mucin. I am interested to study protein-sugar interaction with some biophysical techniques. Can any one suggest...

09 July 2015 4,341 4 View

After purifying my protein it gets easily degraded when I am concentrating it. What would be the solution?

My protein of interest is actually of 39kDa and after purification I am getting at 66kDa and through MS/MS analysis it is proven that the band is actually the same 39Kda protein. My lysis buffer...

01 July 2014 9,099 13 View

How to perform gromacs MD simulations of a protein to get the atomistic details of its unfolding at different temperatures?

I want to perform a MD simulation of a protein at different temperature to check its stability and unfolding at different temperature (300K, 310K, 320K etc.).

30 June 2014 5,801 4 View