How to increase the efficiency of transferring large molecular weight proteins ~265 kDa for western blot analysis?

I am trying to analyse acetyl CoA carboxylase (ACC) protein (~265 kDa) using Biorad Criterion gels and Nitrocellulose membranes. I have already tried separating it on a 7.5% gel with 4% stacking...

17 June 2013 6,374 7 View