Dharitree Samal

2 Questions 4 Answers 0 Followers

Questions related from Dharitree Samal

What is the standard protocol for assessing the efficiency of primers used in qRT PCR?

I have been using the serial dilution method of analyzing the efficiency of Primers designed, via Standard curve. For the assay I take mixture of variety of cDNA's but every time the efficiency...

15 April 2023 4,697 7 View

My iPSCs(induced pluripotent stem cells) NIH5 cell line are not getting revived consistently.What could be the reason?

Since 2 weeks I am trying religiously to revive my iPSC cell line ,but to my dismay the cells are not getting revived.This did not happened earlier.For the attachment of cells I am using...

06 June 2022 9,500 3 View