Dhanasekhar Reddy

4 Questions 2 Answers 0 Followers

Questions related from Dhanasekhar Reddy

Why my PCR bands very faint??

I want to do cloning , for that i need to get the whole gene which is 1.7 kb size. but im getting very faint bands in PCR.The DNA sample is from bacteria and is isolated by using kit. Im using...

06 June 2016 368 2 View

How to purify a protein having theoretical pi of 9.54 using FPLC

iam trying to purify a protein from lactobacillus which is having molecular weight of around 48.96 kDa. the theoretical pi of the protein is 9.54. Anyone please suggest me which buffer i hav to...

02 February 2016 8,041 3 View

Can someone help me with protein isolation?

I want to determine ideal pH of my buffers to isolate my protein. The isoelectric point of my protein is 9.54 (from expasy). What must be the pH of my buffers to make the purification go smoothly?

10 October 2015 5,312 4 View

What are the methods to isolate surface layer proteins?

I am trying to isolate the SlpA (Surface Layer Protein A) protein from Lactobacillus brevis with the Laemmli cell lysis buffer. but i am unable to get protein band in SDS PAGE run at the 44.31...

05 May 2015 4,277 5 View