What power conditions (volts or mA) should I use in Western blotting for transferring a 25kDa protein onto a 0.22 micron nitrocellulose membrane?

Initial transfer performed overnight (16hr) at 30V failed because small and mid sized proteins (smaller than 100kDa) transferred right through the membrane (as evident from Ponceau staining of the...

06 June 2013 6,605 20 View