Devendra Vaishnav

7 Questions 15 Answers 0 Followers

Questions related from Devendra Vaishnav

How can I measure total phenol and total flavonoid in different extracts?

I have prepared crude extract from the plant material and then fraction it with different solvent. How can I calculate the total flavonoid and total phenol in each fractions. First I extract the...

20 February 2015 7,507 8 View

How does the Comet assay (Single cell electrophoresis) work?

I am working on plant extraction and I want to check the DNA protecting activity of my extracts. How does the comet assay work? Is there any other alternative to comet assay? Can anyone provide or...

26 April 2014 6,343 4 View

What is the basic principle for selecting mobile phase in preparative column chromatography?

I am trying to separate the phytochemicals from the crude extract. What parameters should I consider for the mobile phase selection for column chromatography?

09 January 2014 1,277 10 View

What is the normal range of insulin in rats and in STZ treated rats?

I am working on plant extracts active against type I diabetes. I would like to know the normal range of Insulin in normal rats and STZ treated rats.

12 December 2013 3,949 4 View

How to isolate fatty acids from the plant seed oil?

I have collected the hexane extract having positive fatty acid test, I'd like to separate the fatty acids from it.

17 October 2013 2,768 6 View

How can I select the plant active against diabetes?

On what bases I can select the plant against diabetes? Is there any strategy for selecting plants for specific diseases.

08 October 2013 1,853 6 View

Which method is best suited for estimation of Cox II in diabetic rats, ELISA or Western blotting?

Kindly help me to choose the appropriate method for estimation of Cox II in diabetic rats, ELISA or western blotting.

29 September 2013 10,006 19 View