Devanshu Mehta

4 Questions 8 Answers 0 Followers

Questions related from Devanshu Mehta

How much time is sufficient for removing most of the salt from the protein containing buffer using dialysis?

Protein is in PBS (40mM phosphate buffer and 100mM NaCl). Dialysis performed at 4 degree Celcius. Thanks!

07 July 2019 3,844 5 View

Why and how does the excitation maximum and emission maximum of Thioflavin-T (ThT) changes during the lag phase of amyloid fibril process?

Shift of ThT excitation maximum (from 385 nm to 450 nm) and its emission maximum (from 445 nm to 482 nm) occurs only upon binding to amyloid fibrils ( doi:10.1016/j.bbapap.2010.04.001). And we do...

05 May 2019 1,388 5 View

Why the column pressure of Superdex 75 10/300 GL has increased to 1MPa even at a flow rate of 0.01ml/min after 0.5M NaOH wash?

Have been facing problem related to the column pressure from quite some time. Started the column clean-up procedure using 0.5M NaOH. Firstly, washed it twice the column volume with milliQ. Then,...

02 February 2018 7,612 10 View

Insilico Protein peptide docking

Hi, I have following queries: 1. How to find the binding energy value(s) for a protein peptide docked structure(s)? Which software(s) could be used and how? IF NOT. 2. Which software(s) can do...

01 January 1970 9,204 5 View