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Questions related from Devanshu Mehta
Protein is in PBS (40mM phosphate buffer and 100mM NaCl). Dialysis performed at 4 degree Celcius. Thanks!
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Why can't the infra-red beam be made to fall on the sample directly and then allow a measure of molecular absorption and transmission by the detector in IR spectroscopy? Why use an Interferometer?
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Have been facing problem related to the column pressure from quite some time. Started the column clean-up procedure using 0.5M NaOH. Firstly, washed it twice the column volume with milliQ. Then,...
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In general, in a ligand-protein binding study, is there a limit to what percentage of a ligand can be used relative to the final reaction volume? For instance, a ligand is dissolved in buffer A...
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