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I am cloning a small fragment into my vector harboring Cas9 gene using BplI enzyme for restriction digest. I linearized my vector and treated with rSAP before performing gel extraction to excise...
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My gene of interest is approximately 2.2 kb long coding for 405 amino acids in length. As this is my first attempt in using CRISPR-Cas9, I am targeting the N-terminus in attempts to delete DNA...
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