Despoina Gavriilidou

4 Questions 5 Answers 0 Followers

Questions related from Despoina Gavriilidou

Why my fibroblast-secreted fibrils are very thin?

Hi everyone, I am trying to produce extracellular matrix using NIH-3T3 cells. According to previous literature the cells need to be quite old, around 20 passages before you use them to produce...

05 February 2016 2,127 1 View

Any advice on the detachment of cells from matrix?

Hi there, I incubate cos-7 cells for 90min with extracellular matrix (matrix that has been produced by NIH-3T3 cells) and I need the cos-7 cells and use them for signaling assays. Any idea how to...

20 November 2015 990 9 View

Why dead NIH-3T3 cells stay attached to coverslip?

I cross-link coverslips with gelatin, glutaraldehyde and ethanolamine and then I seed them with NIH-3T3 cells. They grow fine but when I lyse them they remain on the surface of coverslip-Why? How...

10 June 2015 4,285 2 View

Why do NIH-3T3 cells die after incubation with ascorbic acid-containing medium?

Hi everyone, I am growing NIH cells in FBS and are currently in passage 15. I want to use them to promote collagen I secretion. So, I seed them in wells and after overnight incubation replace...

22 April 2015 4,561 3 View