3 Questions 2 Answers 0 Followers
Questions related from Despoina Gavriilidou
Hi everyone, I am trying to produce extracellular matrix using NIH-3T3 cells. According to previous literature the cells need to be quite old, around 20 passages before you use them to produce...
05 February 2016 2,092 1 View
I cross-link coverslips with gelatin, glutaraldehyde and ethanolamine and then I seed them with NIH-3T3 cells. They grow fine but when I lyse them they remain on the surface of coverslip-Why? How...
10 June 2015 4,233 2 View
Hi everyone, I am growing NIH cells in FBS and are currently in passage 15. I want to use them to promote collagen I secretion. So, I seed them in wells and after overnight incubation replace...
22 April 2015 4,519 3 View