Deodatus Kardo

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Questions related from Deodatus Kardo

Why I get more than 1 band when performing colony PCR by using KAPA?

Currently I do pENTR D TOPO cloning. I transform my construct (plasmid containing my interest gene) into E. coli DH5 alfa and then checking the result by doing colony PCR using KAPA. I use M13...

11 November 2017 3,531 3 View

Is it possible to use Sybr Safe DNA staining to visualize single strand RNA in gel electrophoresis?

Does anyone know the best way to visualize ssRNA in gel electrophoresis?

07 July 2016 5,070 5 View

Why is NASBA only good for amplifying RNA below 250 bp?

I have read one journal "Characteristics and Applications of Nucleic Acid Sequence-Based Amplification" by Deiman et al., 2002. Is it possible to "upgrade" NASBA so it can be used to amplify...

07 July 2015 6,249 2 View

Does anybody have experience in using BioEdit?

I have an alignment FASTA file of Dengue virus genome from NCBI. Based on that alignment i construct my own primer. After that i added my own primer sequences in the FASTA file of Dengue virus...

07 July 2015 4,109 3 View