Dennis Pägelow

2 Questions 3 Answers 0 Followers

Questions related from Dennis Pägelow

Which is the prefered method for dissociation of neonatal murine brain tissue?

Hi everyone, I would like to hear your opinions and experiences about dissoctiaton and enzymatic digestion of murine neontal (~P2-P8) brain tissue.Our goal is to obtain cell suspensions for flow...

02 March 2017 8,076 2 View

Can activated microglia increase their CD45 expression level from CD45low to CD45high?

Hey everyone, I have the following situation in a murine bacterial CNS infection model: We gated whole brain cell suspensions for CD45/CD11b and afterwards for CX3CR1/Ly6C. We observed one...

09 September 2016 1,491 2 View