Deniz Cansen Kahraman

3 Questions 5 Answers 0 Followers

Questions related from Deniz Cansen Kahraman

Does expired PaxGene tubes have effect on the cfDNA yield or quality?

I have been using a set of PaxGene tubes to collect blood samples from which I have been isolating cfDNA for NGS analysis. I need to collect more blood samples, but I noticed that the expiration...

10 March 2020 4,085 1 View

Which concentration measurement is reliable for cfDNA? Nanodrop or Qubit?

I am trying to isolate cfDNA from plasma samples (2-3 ml) for NGS. I get around 15 ng/ul concentrations of cfDNA from nanodrop but 10 times lower conc (1.4 ng/ul) from qubit dsDNA HS assay. Why...

23 July 2018 7,290 3 View

Can anyone tell me the ingredients of liver cancer stem cell media?

I am currently isolating liver cancer stem cells from HCC cell lines by using magnetic cell sorting system. But after sorting I want to keep them in their undifferentiated state for my experiments.

27 October 2014 9,735 3 View