Denis A. P. Moura

3 Questions 2 Answers 0 Followers

Questions related from Denis A. P. Moura

How to choose a donor plasmid for HDR, with puromycin resistance?

Hello. I have a CRISPR knockout experiment designed for FAC-sorting my transfected cells (GeneArt OFP kit). Due to some events I may not be able to use the FACS anymore. Now I'm trying to use...

04 April 2016 1,964 3 View

What method can I use to investigate phosphate transport in living human cells?

I'm conducting an analysis of a phosphate transporter and I want to measure its transport capacity in human cells. I cannot use radioisotopes in this experiment.

09 September 2015 10,013 1 View

What is the current most reliable protein structure prediction (ab initio/homology based) program?

I have been wondering what would be the current most reliable protein structure predictior from amino acid sequence. I`m interested in both ab initio and template based predictions. You may answer...

01 January 1970 6,821 5 View