How to stimulate NLRP3-mediated IL-1beta production in macrophages?

We are trying to reproduce a model of MLRP3 activation. Based on literature protocols, we prime peritoneal murine C57bl/6j macrophages with 1 ug/ml LPS in DMEM or RPMI1640 for 3 hr., then incubate...

14 October 2022 9,434 1 View