Demagi Herath

3 Questions 5 Answers 0 Followers

Questions related from Demagi Herath

Do methylated primers amplify unmethylated DNA?

I used bisuflite converted, fully methylated DNA, bisulfite converted fully unmethylated DNA and unconverted DNA controls from Qiagen and tested out methylated and unmethylated primers. The...

03 September 2023 2,797 0 View

Protein expression of Gal-genes in yeast?

Hi, I was carrying out Galactose induction experiments to determine protein stability. However, one of my wild-type strains expressed only a trace amount of proteins, which was unexpected as...

23 October 2018 6,949 4 View

Quantitative Methylation Specific PCR - Primer Design

Hi there! Can I please know when designing primers for qMSP, can we theoretically have zero mismatches or is there a certain number of mismatches that can be present before ordering the primers?

01 January 1970 8,571 4 View