Delong Zhang

3 Questions 3 Answers 0 Followers

Questions related from Delong Zhang

Taqman probe qPCR standard curve ? Expand the linear range in serial dilution standard curve?

Question about expanding the linear range for my standard curve dilution in Taqman probe qPCR? I am doing a 4-fold serial dilution using the Taqman probe assay to generate a standard curve...

21 May 2021 4,040 3 View

How to reduce contaminant (230nm) while Using DNAZOL protocol for sperm DNA extraction ?

I add the β-Mercaptoethanol and DTT together with DNAZOL for my lysis buffer, sperm need harsh solution for open them. After washing with 70% ethanol 3-4 times. In the Nanodrop result, I still...

10 March 2021 3,907 2 View

Using ROX and SYBR green for qPCR, how to solve the shoulder problem in the melt curve?

I am using genomic DNA from sperm as the template, try to do sex-ratio relative quantification, when I ran the qPCR, it shows the shoulder in the melt curve, When I ran a gel for the qPCR...

05 March 2021 1,026 4 View