Delhousie Daniel Jambun

7 Questions 15 Answers 0 Followers

Questions related from Delhousie Daniel Jambun

Is it bad for an anticancer agent to kill cancer cells through necrosis?

I'm currently working on a compound that I had isolated which exhibited anticancer activity. It has a therapeutic index of more than 3 and so I'm interested to know its mechanism of action. Based...

04 June 2017 1,028 10 View

Positive cell proliferation on HaCaT but not 3T3?

I'm working on a wound healing project and currently trying to see whether my extract has positive effect on cell proliferation. The two cell lines that I'm using are HaCaT keratinocyte and 3T3...

11 May 2017 5,872 1 View

In DEPT experiments, why do some peaks appear positive in DEPT-45 and DEPT-90 but negative in DEPT-135?

I noticed that in the DEPT experiment that I did, some peaks show up in all three DEPTs (45, 90 and 135). If I'm not mistaken, DEPT-90 is supposed to show only CH peaks while in DEPT-135, CH peaks...

16 July 2015 4,485 5 View

Can bacteria mutate to become resistant to phyotchemicals that act directly on their membrane by "dissolving" them?

One example of such a phytochemical might arguably be dodecenal. Due to the nature of this compound having a lipophilic tail and hydrophilic head, the tail can sit itself in the bacteria membrane...

12 November 2014 8,359 5 View

What could explain the differences between my specifically obtained positive and negative mode mass chromatogram of the same pure compound?

In the attempt to identify a pure compound that I had isolated from the plant that I'm studying, I conducted ESI-TOF mass spectrometry analysis using positive and negative modes. In addition to...

09 November 2014 551 13 View

What are the optimal scanning electron microscopy parameters when viewing bacteria?

I would like to evaluate the effects of several antimicrobials on bacterial cell morphology. Can anyone suggest what are the optimal settings for the scanning electron microscopy? The parameters...

13 September 2014 2,482 8 View

Would the use of sub-MIC affect the degree of biofilm formation?

When dealing with plant crude extracts, antimicrobial and/or antibiofilm compounds might be present in them. So, what we usually do is that we test for MIC of the crude extracts and then if the...

05 August 2014 3,240 12 View