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I have been trying to conjugate DBCO (Dibenzocyclooctyne) modified quantum dots to azide modified oligonucleotides. I havent been able to get this to work well. Can anyone recommend a protocol for...
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I conjugated DBCO modified quantum to azide modified oligonucleotides using SPAAC click chemistry, I have been trying to use HPLC SEC to separate unbound azides and characterize the conjugation....
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