Deepti Harinder

9 Questions 22 Answers 0 Followers

Questions related from Deepti Harinder

Is there any particular advantage in using a mixture of nitrogen and carbon dioxide versus absolute nitrogen for anaerobic fermentation?

I am working on understanding global transcriptional regulator (FNR, ArcA, IHF) effect on E coli K12 both from genotypic and phenotypic point of view under anaerobic conditions. Initially I had...

13 January 2016 138 9 View

How can I calculate the concentration of HCO3 ions in media when it is incubated in air enriched with 1%CO2?

I want to calculate the amount of CO2 that will be dissolved in my media if I incubate it in air containing 1%CO2. Can someone please help me with this calculation?

24 September 2014 1,579 2 View

Can anyone suggest ways of improving 13C labelling of TCA intermediates?

I am interested in studying the labelling pattern of a cyanobacteria using 13C. I do a steady state labelling with uniformly labelled 13C-NaHCO3 and draw out samples at different times points...

17 July 2014 8,620 13 View

Can someone give me a protocol for extraction of organic acids for GC-MS analysis from Synechococcus cultures?

I would like to analyze organic acids from Synechococcus cultures using GC-MS analysis. I am currently following a protocol which uses Methanol-Chloroform-Water (MCW) for extraction and then...

19 June 2014 6,504 0 View

Where can I get the mass spectra and retention times for TMS-derivatised succinate, formate, malate and citrate?

I want to get the retention time and mass spectra for derivatised standard organic acid solutions of fumaric acid, malic acid, succinic acid and citric acid. I have done a GC-MS of a solution...

16 June 2014 7,424 7 View

Does boiling of protein samples affect their estimation by Bradfords Assay?

I want to know whether I can use Bradfords Assay to estimate protein from samples that have been subjected to boiling for lysing the cells.

14 June 2014 5,393 0 View

What is the advantage of using FAME over free fatty acids for estimation using GC?

Analysis of fatty acids in the free form instead of fatty acid methyl esters is relatively simpler and quicker. Then why is FAME-GC preferred over free fatty acid analysis by GC? What are the...

12 June 2014 619 4 View

Can I use a speed vac evaporator for drying my samples (1ml) for metabolite analysis

We have always used N2 purging to dry up the samples. However, since we do not have an automated instrument for the N2 purging, we do it manually, one sample at a time and this is becoming very...

11 June 2014 5,026 11 View

What is a simple method to estimate the total lipids from cyanobacterial culture?

Can someone suggest a simple method to estimate the total lipids from cyanobacterial culture? I have read up on a couple of gravimetric methods that would extract and estimate the total lipids,...

11 June 2014 7,517 7 View