Why control values of DCFDA stain is high compare to treatment with 500 uM OA:PA(2:1) in HEPG2 cells?

Hi, I am doing DCFDA in HEPG2 cells. cells treated with 500uM OA:PA(2:1) overnight. after that 10uM DCFDA stain with Hoechst stain for 30 minutes is performed. cells analysed by plate reader and...

22 November 2017 9,668 1 View