Why HPLC Column doesn't last for more than 500 injections for the analysis of imipenem?

Worked on 2 separate HPLC methods, first one uses 100% of 5mM sodium phosphate pH6.8 and aqueous compatible HPLC column and column oven temperature of 40C. Get resolution between imipenem...

04 January 2017 6,330 5 View