Deepak Saini

2 Questions 1 Answers 0 Followers

Questions related from Deepak Saini

Transfection of large DNA plasmids into HEK293T?

Hi, I have been recently trying to transfect HEK293T with a large plasmid (http://www.addgene.org/53191/) which is around 15 Kb. I am using the PEI method to transfect this DNA. My protocol so...

02 February 2020 6,132 3 View

Using RT-qPCR to validate CRISPR ko trouble?

Hi, I recently made a mutant cell line by excising an exon from the gene, DNMT1, using CRISPR-CAS9. I isolated a single cell population that has this mutation and confirmed the mutation using PCR...

01 January 2020 5,031 3 View