Deepak Kumar

3 Questions 7 Answers 0 Followers

Questions related from Deepak Kumar

Why there are multiple bands in Western blot after protein expression in insect cells?

Hi, I recently used an old P0 Baculovirus (stored at 4C for 8-9 months) to produce a viral glycoprotein (37kd). My previous expression for this protein using SF9 cells was always very low so I...

02 February 2020 3,806 6 View

What is the best way to solubilize an insoluble protein produced in Baculovirus expression system for his-tag purification?

Hi, I have produced a 37kda viral protein using Baculovirus expression system (FlashBac Ultra)/insect cells. It is a glycosylated protein and contains a transmembrane region at N-terminal....

06 June 2019 6,642 8 View

Can anyone suggest protocol for long term storage of Campylobacters?

We use 20% Glycerol stock for Campylobacter isolates and store at -80C but fail to revive after 3 months. Please share your experiences.

02 February 2017 5,066 3 View