Deepak Saini

2 Questions 1 Answers 0 Followers

Questions related from Deepak Saini

What is the best PCR strategy to genotype large deletions?

Hello, I currently setting up an experiment to study the role of a set of genes that span 150 Kilobases in my cell line. So I plan on generating a 150 KB deletion by nucleofecting Cas9 with 2...

04 June 2020 3,322 8 View

Transfection of large DNA plasmids into HEK293T?

Hi, I have been recently trying to transfect HEK293T with a large plasmid (http://www.addgene.org/53191/) which is around 15 Kb. I am using the PEI method to transfect this DNA. My protocol so...

15 February 2020 4,696 4 View