Deepak surya Dadi

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Questions related from Deepak surya Dadi

What might be the reason for smear formation in agarose gel "while extraction of DNA from pre performed agarose gel"?

the concentration of DNA sample before extraction is 4.16 u gm/ml, after extraction of this DNA from agarose gel the concentration was found as 4.15 u gm/ml hence,the recovery is 99.7%. But, after...

21 August 2015 9,099 8 View

How could the yield of RNA be increased a bit by adding "glycogen" while extraction of RNA from animal cells?

I came to knew that while extraction of RNA from animal cells by adding "glycogen or any other linear carrier" the final yeild of RNA has increased a bit. So, can any one give me the brief...

21 August 2015 221 19 View

How can I stop "false positive (+) results" while screening my rDNA expressed colonies from other really unexpressed colonies?

I am using pBR322 as E. coli cloning vector which as we know having marker sites ampicillin and tetracycline, after division of host cells I transferred these colonies to ampicillin- and...

19 August 2015 7,650 4 View