Deepak surya Dadi

5 Questions 3 Answers 0 Followers

Questions related from Deepak surya Dadi

Can any one suggest a way to remove the stop codon at the end of ORF of my nucleotide sequence?

I want to insert my GOI in the MCS region of p28a vector, it has his tag so screening is very easy for me. But my GOI has stop codon at the end. So, definitely it will stop the expression of his...

08 March 2016 5,655 2 View

What might be the reason for smear formation in agarose gel "while extraction of DNA from pre performed agarose gel"?

the concentration of DNA sample before extraction is 4.16 u gm/ml, after extraction of this DNA from agarose gel the concentration was found as 4.15 u gm/ml hence,the recovery is 99.7%. But, after...

21 August 2015 9,113 8 View

How could the yield of RNA be increased a bit by adding "glycogen" while extraction of RNA from animal cells?

I came to knew that while extraction of RNA from animal cells by adding "glycogen or any other linear carrier" the final yeild of RNA has increased a bit. So, can any one give me the brief...

21 August 2015 229 19 View

How can I stop "false positive (+) results" while screening my rDNA expressed colonies from other really unexpressed colonies?

I am using pBR322 as E. coli cloning vector which as we know having marker sites ampicillin and tetracycline, after division of host cells I transferred these colonies to ampicillin- and...

19 August 2015 7,666 4 View

Why plant cells have capacity to express more than one trans gene , allowing in it the delivery of multiple antigens for repeated inoculation?

I have been studying about how to produce vaccines and hormones of both prokaryotic and eukaryotic origin mainly by bio-pharming and transgenic plant technology. So, your answers will help me to...

18 August 2015 2,724 3 View