Why I am getting an extra band other than the released insert upon double digestion of a Recombinant plasmid?

I have cloned a 1 kb gene into a 5.3 kb vector (in between BamH1 & Xho I) and screening of the recombinant plasmid has been done by colony PCR using gene (insert) specific primer where I am...

22 June 2023 3,011 3 View