Deelaka Wellappili

3 Questions 1 Answers 0 Followers

Questions related from Deelaka Wellappili

When serum starving HeLa cells, what percentage of FBS should I use in DMEM, if any at all?

Should I use 1%FBS in DMEM instead of the normal 10%? Or should I use no FBS at all?

15 May 2018 3,546 4 View

How am I getting two RNA bands after doing In vitro transcription?

I am doing an IVT of guide DNA sequences. My template DNA is ~120 bp, but after the T7 promoter, there are only ~100bp. But after I do the IVT and run on a gel, I get two separate bands. I am...

04 November 2016 1,577 5 View

Why would there be differences seen in gene expression measured using RNA-Seq and enzyme assays for reporter gene fusions?

I am studying the expression of certain genes when large sections of the bacterial genome is deleted. I am comparing the expression of reporter genes fused to the genes being studied, with results...

29 March 2016 8,621 6 View