Deekshi Angira

3 Questions 3 Answers 0 Followers

Questions related from Deekshi Angira

Is it possible for a protein to form dimer when expressed in bacteria ?

The protein that I am working on is a 16 kD protein. When I am trying to express this protein in E. coli and performing SDS PAGE I am getting a very faint band at 16 kD but at the same time I am...

12 April 2015 1,514 10 View

Is it possible that a very low amount of protein is expressed in uninduced culture too ?

I induced a culture (Rosetta gami pLysS) with IPTG ( 0.5 mM and 1 mM) at 37 degrees. Together with it I also kept a control ( uninduced). After lysis, when I run SDS-PAGE gel, I get a band at...

22 January 2015 5,508 4 View

Is there any possible method to prevent our protein from going into inclusion bodies after expression, apart from using solubility tags?

The protein that I am working on has a tendency to form inclusion bodies. So, I want to know that there is anything that we can add or any precaution that we can take during protein expression...

06 January 2015 5,604 11 View