G-tailing or G/I-tailing for poly (A) tail (PAT) assay?

I wish to perform a PCR-based poly (A) tail length (PAT) assay. After isolating total RNA from cells, I want to ligate their 3' ends in vitro with a 5' phosphorylated DNA adapter. Why do some...

21 June 2022 5,838 0 View