Debra Brunson

3 Questions 1 Answers 0 Followers

Questions related from Debra Brunson

Should my alpha be set to .05 or .01?

I am wondering under what circumstances is it more appropriate to use an alpha value of .01 instead of the standard .05 (using a T-test for equal or unequal variances). I have some data in which...

06 November 2016 5,579 9 View

Does anyone have advice on how to increase transformation efficiency in a high capsule producing strain of Klebsiella pneumoniae?

I am working with ATCC 43816 Klebsiella pneumoniae and am having extreme difficulty in creating knockouts using a lambda red recombination system. This system has been successful in other strains...

22 April 2016 5,988 2 View

Suggestions for uronic acid quantification assay

I am trying to use the protocol outlined by Dominenco to extract capsular polysaccharides from Klebsiella pneumoniae. The samples are then used to determine uronic acid concentration as an...

19 February 2016 908 3 View