What am I doing wrong when in a PCR-products electrophoresis gel the only thing I see are primer dimers, no desired band even in positive control?

I am trying to amplify a fragment of CMV genome from a dried blood spot (Guthrie card) but all I see in agarose electrophoresis gel are primer dimers, no expected band even in positive control. It...

13 May 2016 7,295 16 View