Debomita Bhattacharya

2 Questions 2 Answers 0 Followers

Questions related from Debomita Bhattacharya

Why is there a significant difference between IC50 in enzymatic assay and KD of ITC?

When checked in enzymatic assay, a compound with IC50 37 nM showed a KD value of 20 µM against ITC. How can this discrepancy be explained?

18 July 2023 3,496 1 View

The IC50 value of racemic is 2.4 μM,for R is 1.1 μM for S is 0.5 μM. How to explain the difference in activity?

I have synthesized a racemic compound and its two enantiomers. The IC50 value of racemic compound is 2.4 μM, for R is 1.1 μM and for S is 0.5 μM against the target protein. How to explain the...

09 May 2023 1,591 1 View