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Questions related from Debjyoti Dutta
I have sequenced a 7 kb part of frog mtDNA and have generated a bam file of the sequence. In IVG, I can visualize manually the per base read coverage but I need this information in an excel format...
10 June 2018 9,473 2 View
I have generated a pileup of Illumina Miseq NGS data in IGV by mapping the reads onto the reference sequence reconstructed from the reads in order to check how well the sequencing has worked. The...
23 October 2017 9,983 3 View
I have sequenced partial mitochondrial genome of Philautus sp. and have generated a consensus representing the reconstructed genome from the reads. There are many single nucleotide variants...
13 July 2017 405 2 View
I have sequenced the rhodopsin gene of philautus in Illumina genome analyzer which gives paired end reads. I obtained 10 contigs from the paired reads using Velvet 1.1.10 and then scaffolded the...
10 June 2017 5,156 1 View
I am using Velvet_1.2.10 assembler to obtain de novo sequence of an amplicon. I have obtained contigs given by velvetg containing 10 nodes and n50 value of 1023. How do I know the order and...
05 June 2017 2,070 2 View
At the annealing step of PCR it is the oligonucleotide primers that binds to the single stranded template and initiates extension. But do the two single strands also anneal among themselves?
18 June 2016 4,371 3 View
I am trying to design primers for amplifying certain genes across the amphibian taxa. I know that the lower limit for designing primers is 18 bases for reasons of specificity. However, what could...
02 May 2016 1,592 5 View
