Debjyoti Dutta

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Questions related from Debjyoti Dutta

How to obtain per base read coverage of a bam file in excel format?

I have sequenced a 7 kb part of frog mtDNA and have generated a bam file of the sequence. In IVG, I can visualize manually the per base read coverage but I need this information in an excel format...

10 June 2018 9,468 2 View

How to obtain information as a text file from NGS pileup data in IGV?

I have generated a pileup of Illumina Miseq NGS data in IGV by mapping the reads onto the reference sequence reconstructed from the reads in order to check how well the sequencing has worked. The...

23 October 2017 9,977 3 View

Why don't single strands anneal among themselves during annealing step of PCR?

At the annealing step of PCR it is the oligonucleotide primers that binds to the single stranded template and initiates extension. But do the two single strands also anneal among themselves?

18 June 2016 4,364 3 View

What are the disadvantages of designing a very long primer (50 bases>)?

I am trying to design primers for amplifying certain genes across the amphibian taxa. I know that the lower limit for designing primers is 18 bases for reasons of specificity. However, what could...

02 May 2016 1,586 5 View